DRIED BLOOD SPOT
a)difinition of DBS;-is a whole blood collected on a filter paper and dried which allows for easier transportation of the specimen.
b)Requirements during prepairing DBS
1)Blood collection cards(filter paper)-is a paper like that are used in collecting blood.
2)Sealable plastic bags-is a plastic bag used to store or collect all the requirement that are used in DBS.
3)Glycine weighing paper-it stands between DBS cards for transporting/prevent coss contamination.
4)Desiccant packs-when transporting from one place to another it helps to remove the excess moisture.
5)Humidity cards-is a card on which a moisture sensitive chemical is impreginated that can change colour.also help to maintain moisture during transportation.
6)Sharp containers-used for deposing the sharp instuments after work.
7)Alcohol swab-used to clean the finger before pricking and after pricking.
C)Important tips for drying DBS.
1)Avoiding touching or smearing the blood spots becouse it may lead to exploding of diseases in case the child has any diseases.
2)it must dry completely before packagein order to be in safe order.
3)heat is not allowed during drying process becouse it will deteriorate DBS.
4)It must be away from direct sunlight becouse the light can give negative results.
5)allow the specimen to fully air dry horizontally as it is adviced.
D)How to pack DBS storage after drying is complete.
1)appropriately stock DBS
-Glycine weighing paper must be placed on DBS to pevent cross contamination.
2)insert into sealable plastic bags
-Bags which are big in size must be avoided becouse cards will be shuffling inside.
3)Desiccant packets shouold be added
-Desiccant save the drying agent,some need more or fewer depending on environment and storage conditions.
-humidity cards must be recharged before use and if a drying oven is not available,cards and excessivenumber of desiccant must be placed.
E)How to pack DBS for shipping.
1-Insert bundled DBS into rip resistant envelope.
2-appropriate documantation with shipment must be included.
3-both into brown envelope and seal for shipping must be inserted
F)How to store DBS
1-it is acceptable to store DBS in a styrofoam box
2-avoid leaving in a motor vehicle,sun and heat will deteriorate DBS
3-spots in an mulfuctioning refrigerator where watermay drip on a soak the spots must be avoided.