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THICK BLOOD SMEAR PREPARATION FROM FINGER PRICK Purpose This procedure provides instructions for preparation of thick blood smears for hemoparasites from finger prick. Materials Reagents Supplies •Absolute alcohol •Cotton wool •Gauze •Protective gears •Alcohol swab •Jar •Wooden applicator •Drying rack •Blood lancet •Sharps container Sample Whole blood Special Safety Precautions All specimens must be regarded as potentially infectious. Refer to Infection Prevention and Control Guidelines for additional safety considerations. Quality Control Refer to the Standard blood smear template. Procedure Follow the activities in the table below. Step & Action 1.Label the slide with patient ID number using permanent marker 2.Clean the ring finger by using alcohol swab and allow to dry 3.Firmly press sterile disposable lancet to puncture the finger tip  4.1 Wipe off the first drop of blood and slightly apply gentle pressure.

Carbohydrate metabolism Definitions (15 minutes) ACTIVITY: Define the following terms (Glycolysis, Glycogenesis, Glycogenolysis, Gluconeogenesis, Glucose Tolerance) What is Carbohydrates Metabolism? Definitions: ·          Glycolysis i s the process whereby glucose molecule is hydrolyzed in the body in order to obtain ATP and Pyruvate. ·          Glycogenesis; the process of glycogen formation which   takes place in the liver and stored there. ·          Glycogenolysis is the process of splitting glycogen. Glycogen molecules do not remain in the cell permanently but are eventually broken apart (hydrolysed). Glycogenolysis alone can maintain homeostasis of blood glucose concentrate for only a few hours, since the body can store only small amount of glycogen. Gluconeogenesis ·          Gluconeogenesis means the formation of the new glucose in the sense that it is made from protein or less frequently from the glycerol or fats