THICK BLOOD SMEAR PREPARATION FROM FINGER PRICK
This procedure provides instructions for preparation of thick blood smears for hemoparasites from finger prick.
Special Safety Precautions
All specimens must be regarded as potentially infectious.
Refer to Infection Prevention and Control Guidelines for additional safety considerations.
Refer to the Standard blood smear template.
Follow the activities in the table below.
Step & Action
1.Label the slide with patient ID number using permanent marker
2.Clean the ring finger by using alcohol swab and allow to dry
3.Firmly press sterile disposable lancet to puncture the finger tip
4.1 Wipe off the first drop of blood and slightly apply gentle pressure.
4.2 Place a small drop of blood at the center of pre-cleaned scratch free, labeled glass slide
5.By using a corner of another slide or an applicator stick spread the blood drop in a circular pattern making about 1.5cm2 Avoid making too thick smears
6.Allows the blood smear to air dry protected from direct sunlight, dust and insects
7.Transport the dried blood smear accompanied with the patient request form within an hour
Health Laboratory Safety and Waste Management manual
STOOL CULTURE COLLECTION PROCEDURE
This procedure provides instructions for the collection of a urine specimen.
Reagents, Supplies, Equipment
•Screw-capped stool containers
•Permanent marking pens
Special Safety Precautions
All specimens must be regarded as potentially infectious
Refer to the Specimen Management and Waste Disposal Manual
Follow the activities in the table below to collect a urine specimen.
1.Collection, Labeling and Registration
1.1.Properly identify the patient with at least the Patient’s name, PID, date of birth, and Doctor’s name.
1.2.Wash hands with soap and water
1.4.Have all the needed specimen collection tools on hand Refer to specimen test catalog.
1.5.Explain the specimen collection procedure to the patient
1.5.1.Provide patient with a wide-mouth plastic, screw-capped stool container
1.5.2.Instruct patient to deposit approximately one spoonful (Spoon provided) into the screw-capped stool container.
1.5.3.Instruct patient to seal the specimen container tightly.
1.5.4.Ensure the stool in the container is sent to the laboratory.
1.6.Label each specimen immediately with identifying information – patient identification (PID), date and time of collection.
1.7.Fill out lab request/report form for each specimen with date, time and initials of collector.
1.8.Check that PID on the specimen container matches with that on the lab request/report(Check specific name of form) form.
NOTE: This labeling and matching is the primary responsibility of the collector and must be done carefully and precisely.
1.9.Place collected specimens in a transport container at an appropriate temperature, awaiting transport to the laboratory.
1.10.Dispose of gloves and wash hands after each specimen collection/handling.
1.11 Enter specimen information into specimen register book
Practical Laboratory Manual for Health Centers in East Africa, AMREF, Jane Carter Orgenes Lema 1994, pg. 170-171.
District Laboratory Practice in Tropical Countries, Monica Cheesebrough Part I, 1998,