THE PREGNANCY

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Pregnancy Test

Prepared by ntangakeneth.blogspot.com
ADMLS Microbiology/ Immunology
 Reagents, materials, and types of samples
Reagents and Materials
Disposal bucket
Pipettes
Test kit reagents
Types of Sample
Spot (at the moment) urine sample (about 20 ml).  An
 early morning urine sample is more sensitive. 
Centrifuge turbid samples prior to testing. Note the
 storage requirements for urine samples in the package
insert.

 Safety precautions, procedure with limitations, quality control, and reporting results
Safety Precautions
Appropriate biosafety practices should be used when handling specimens and reagents. Refer to Laboratory Safety and Waste Management Module for more detailed information.
Procedure
Refer to the kit manufacturer’s instructions.  Every laboratory should prepare its own method sheet based on the type of kit used

Limitations
If a first test is negative, the test can be repeated after 5 days.
Store reagents at the correct temperature according to the manufacturer’s instructions. 
Check carefully the expiry dates on the test kits.
Check that the catalogue numbers on the reagent containers match the numbers on the kit procedures in the package insert.

Note the precautions listed in the package insert.
Each laboratory must determine the time necessary to perform the procedure (turnaround time).

Quality control
Always use standards and controls according to the manufacturer’s instructions to ensure the test results are reading correctly.
Reporting results
Report as pregnancy test positive or negative
Indicate date and name of technical staff reporting.
Key points
The pregnancy test can be used for more than diagnosing
 pregnancy.
Store reagents at the correct temperature according to
 the manufacturer’s instructions. 
Check carefully the expiry dates on the test kits.
Check that the catalogue numbers on the reagent
 containers match the numbers on the kit procedures in
 the package insert.
References
Cheesbrough, Monica. 1998. District Laboratory Practice
 in Tropical Countries, Part 1. Low-priced edition,
 Butterworth & Co Ltd.
Standard Operating Procedures Essential Laboratory
 Tests (2008), African Medical and Research
 Foundation.

Identify the reagents and materials used for
 pregnancy test.
State the uses of the reagents and materials for the
 pregnancy test.
Perform the pregnancy test according to SOP.

Identification of reagents and materials for the pregnancy test.
Reagent: pregnancy test kit
Materials
Disposal bucket
Pipettes

 Uses of the reagents and materials for the pregnancy
 test

Reagents
Latex particles coated with monoclonal

 antibodies react with human chorionic

gonadotropin (HCG) in urine to give macroscopic

 agglutination.



Materials
The use of the materials will be explained during
 the performance of the procedure.

Demonstration of the pregnancy test according to the

 SOP Demonstrate of the pregnancy test according to
 the SOP.

 Performance of the pregnancy test (20 minutes)
Students perform the pregnancy test according to the
 SOP.
Key points for the pregnancy test
Store reagents at the correct temperature according to
 the manufacturer’s instructions. 
Check carefully the expiry dates on the test kits.
Check that the catalogue numbers on the reagent
 containers match the numbers on the kit procedures in
 the package

Comments

  1. write with us21 March 2019 at 00:36

    Pregnancy test is carried out in order to confirm whether women are pregnant or not. These can be confirmed with the indicative makers that are found in the urine or blood which are about to be sampled states cm hospital
    Gynecologist In Velachery .

    ReplyDelete

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