STREPTOCOCCUS SPECIES
STREPTOCOCCUS
Define
(Streptococcus species)
This
is a Gram positive cocci occurring in chains or pair
List the species of staphylococcus
- Streptococcus
pyogenes(Group A)
- Streptococcus pneumoniae(pneumococci)
- Streptococcus
agalactiae(Group B)
- Streptococcus viridans
- Streptococcus feacalis(Enterococci)(Group
D)
- Streptococcus mitis
- Streptococcus mutans
- Streptococcus sangius
Mention the species of medical importance
Streptococcus
pyogenes
Streptococcus
pneumoniae
Streptococcus
agalactiae
Describe the normal habitat Streptococcus pyogenes
Streptococcus pyogenes
Vegetation
Milk and milk production
Water
commensal are found in the
upper respiratory tract.
Describe the morphology Streptococcus pyogenes
They are Gram positive cocci.
- They occur in chains.
- Are non motile.
- Some strains are
capsulated.
Describe the mode of transmission and pathogenicity Streptococcus pyogenes
Streptococcus pyogenes is transmitted :-
- Inhalation-Air borne contamination arising from
dressing,wound dressing,bed making,talking directly on wound
- Transferable to clean wound from other patients
with septic lesion by contamination of hands,clothing or instruments,
PATHOGENICITY
Streptococcus pyogenes causes:-
Acute sore throat(tonsilitis).
Scarlet fever.
Ear infections(otitis media).
Skin infections.
Septicemia.
Acute glomerulonephritis.
Rheumatic fever(heart valve
damage).
Enzymes and Toxins produced by Streptococcus pyogenes
Enzymes
The above pathogenicity is due
to production/secretion of enzymes and toxins
These enzymes enable the
bacteria to attack an individual and cause a disease or an infection. They
include:-
- Streptokinase.
- Hyaluronidase
TOXINS
Streptolysin ”O” toxin.
Streptolysin “S”toxin.
Erythrogenic toxin.
Describe the laboratory diagnosis for Streptococcus
pyogenes
1.SPECIMENS
- Throat.
- Pus swab.
- Serous fluid -depending
on site of infection.
- Blood for culture.
2.CULTURE
Streptococcus pyogenes produces Beta-Haemolytic
colonies (colonies are surrounded by a clear zone of complete haemolysis) on
BA.
The media used are;-
i.
Blood Agar
ii.
Macconkey Agar.
i)BLOOD
AGAR AND CHOCOLATE AGAR
Small colonies 1-2mm white un color
are produced.
They show Beta-Haemolysis.
.
(ii)MACCONKEY
AGAR
Streptococcus pyogenes doesn’t grow on
Macconkey Agar.
NB:Crystal violet Blood Agar (CVBA)is a
selective media for isolation of Streptococcus pyogenes only.
3.BIOCHEMICAL
TESTS
Streptococcus pyogenes is identified using the following tests:-
Catalase positive
Bacitracin (sensitive)positive
Key Points
Define
Streptococcus
This
is a Gram positive cocci occurring in chains or pair
List
the species of Streptococcus
- Streptococcus
pyogenes(Group A)
- Streptococcus
pneumoniae(pneumococci)
- Streptococcus
agalactiae(Group B)
- Streptococcus viridans
- Streptococcus
feacalis(Enterococci)(Group D)
- Streptococcus mitis
- Streptococcus mutans
- Streptococcus sangius
Describe
the mode of transmission and pathogenicity
Streptococcus
pyogenes is transmitted :-
- Inhalation-Air borne contamination arising from
dressing,wound dressing,bed making,talking directly on wound
- Transferable to clean wound from other patients
with septic lesion by contamination of hands,clothing or instruments,
Describe
the morphology Streptococcus pyogenes
- They occur in chains.
- Are non motile.
- Some strains are
capsulated.
BIOCHEMICAL
TESTS
Streptococcus
pyogenes is identified using the following tests:-
Catalase positive
Bacitracin (sensitive)positive
.
Evaluation
·
List 3 media used
to isolate bacteria
·
Mention 5
diseases that are caused by S.pyogenes
Streptococcus pneumoniae (pneumococci)
Describe the normal habitat Streptococcus pneumoniae
NORMAL HABITAT
S. pneumoniae is part of the normal upper respiratory tract flora but as with
many natural flora, it can become pathogenic under the right conditions (e.g.,
if the immune system of the host is suppressed).
Persons at higher risk for infection are
the elderly, children under 2 years old and persons with underlying medical
conditions including HIV infection and sickle-cell disease.
Describe the morphology Streptococcus pneumoniae
MORPHOLOGY
- Streptococcus pneumoniae, or pneumococcus,
- Gram-positive diplocci
- Are lancet
shaped
- Alpha-hemolytic,
- Occurs in short chains following culture.
- Non motile
- Most strains are capsulated following culture
Describe the mode of transmission and pathogenicity Streptococcus pneumoniae
Streptococcus
is pneumoniae transmitted :-
By inhalation of air droplets that have the
bacteria
PATHOGENICITY
Streptococcus
pneumoniae causes:-
- lobar pneumonia
,Bronchitis,menengitis,
- Bacteremia,Otitit
media,Sinusitis and conjuctivitis.
- These infections affects
the elderly and the young due to low immunity.
- Those infected by HIV have
a greater risk of infection.
Enzymes and Toxins produced by Streptococcus
pneumoniae
Enzymes
- The above pathogenicity
is due to production/secretion of enzymes and toxins
- These enzymes enable the
bacteria to attack an individual and cause a disease or an infection. They
include:- Hyaluronidase
Toxin
- Leukocidin
- Haemolysin
Describe the laboratory diagnosis for Streptococcus
pneumoniae
1.SPECIMENS
Depends on site of infection.
They are:-
- Sputum
- Exudates
- Blood-for culture
- CSF
2.CULTURE
- A facultative anaerobes
- The organism is alpha
haemolytic in 5-10%co2 in BA and Chocolate heated blood agar.
- It grows on:-
- Blood agar
- Chocolate blood agar.
i) BLOOD AGAR
- Colonies
appear translucent
- They
are 1-2mm in diameter.
- Colonies
are Alpha haemolytic(colonies are surrounded by an area of partial
haemolysis with green- brown color. Alpha haemolysis occurs after
over night incubation in co2.
- Colonies
also show ‘draughtsman shape’.
- NB:Pneumococci
shows beta haemolysis after being cultured aerobically.
ii)CHOCOLATE BLOOD AGAR
• Colonies grow well in this
media when if grown in environment enriched with co2 especially in
candle jar.
3.OCHEMICAL TESTS
Streptococcus pneumoniae is:
·
Catalase negative
·
Bile solubility test positive
·
Optochin test sensitive(+ve)
NB:Bile solubility is a test
used to differentiate S.pneumoniae from other streptococci species.
Key Points
Describe
the morphology Streptococcus pneumoniae
MORPHOLOGY
- Streptococcus pneumoniae, or pneumococcus,
- Gram-positive
diplocci
- Are lancet
shaped
- Alpha-hemolytic,
- Occurs in short chains following culture.
- Non motile
- Most strains are capsulated following culture
Describe
the laboratory diagnosis for Streptococcus pneumoniae
SPECIMENS
Depends on site of infection.
They are:-
- Sputum
- Exudates
- Blood-for culture
- CSF
BIOCHEMICAL TESTS
Streptococcus pneumoniae is:
·
Catalase negative
·
Bile solubility test positive
·
Optochin test sensitive(+ve)
PATHOGENICITY
Streptococcus pneumoniae causes:-
- lobar pneumonia
,Bronchitis,menengitis,
- Bacteremia,Otitit
media,Sinusitis and conjuctivitis.
- These infections affects
the elderly and the young due to low immunity.
Those infected by HIV have a
greater risk of infection
Evaluation·
List 2 media used
to isolate bacteria
· Mention 5
diseases that are caused by Streptococcus
pneumoniae
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