PROCEDURE OF ACCESSIONING SPECIMENS



Procedures of Accessioning Specimens

 

Definition of Terms  


·         Accessioning of specimens
  • Is a process of qualifying specimens for laboratory investigation

Criteria’s for accessioning 
·         Request form should be well filled with;
  • Health facility name
  • Hospital Number
  • Name of patient
  • Date
  • Age
  • Sex
  • Address
  • Ward Number
  • Clinical Note
  • Laboratory investigation
  • Time of request
·         Sufficient quantity of specimen
·         Right container
·         Condition of the specimen

 Criteria’s of Rejecting Specimens 
·  Rejecting criteria
  • Quantity is not sufficient
  • Wrong container
  • Long transport time
  • Wrongly or incomplete filled request form 
  • Condition of the specimen is not ideal for the test (haemolysed blood, dried stool, clots,
  • Unlabelled/Mislabeled specimen

·         Example of Specimen Rejection Form.
  • Hospital Registration Number: …………………………Ward / Clinic …....……
  • Patient Names (in full): ……………………………………………………......…..
  • Date Sample Received: ……………………………  Time: ………………......….
  • Description of Problem encountered: (in brief): ..................................................
  • Corrective Action(s) Needed: …………………………………………….....…….
  • Form Completed by: ………………Signature: ……………Date: …...................
  • For Clinic / Ward: Corrective Action taken ……………………………...………
  • Corrective Action taken by:  Name ………………………………………...…….
  • Signature: ………………………Date: …………………Time……………….
  • Return this form to the laboratory with specimen indicating corrective actions taken

·Rejection Codes
1
No  specimen/sample received
13
No specimen/sample ID
2
Quantity not sufficient
14
No specimen/sample ID cannot be established
3
Haemolyzed
15
Specimen/sample ID illegible
4
Lipemic
16
Collection site inappropriate
5
Damaged-broken or leaked in transit
17
Specimen /sample type unacceptable for test
6
Damaged-contaminated
18
No test requisition received
7
Damaged-expired transport media
19
N test ordered on requisition
8
Damaged-improper preservation
20
No collection date requisition
9
Damaged-improper transport media
21
No specimen / sample collection site on requisition
10
Damaged-improper temperature
22
Test not available
11
Damaged-lab accident, unsalvageable
23
Clotted Sample
12
Sample too old



Importance of Accessioning 
·         Importance of Accessioning are
o   Helps to get good results
o   It helps to avoid mixing of specimen
o   Helps track down specimen/results
o   Helps mismanagement of specimen
o   Helps to get results at right time
o   Helps patient to get treatment at right time
o   Helps the laboratory workers to plan their work
o   Helps to process the right specimens
o   It helps to keep records

Procedure of Accessioning Specimen 
·   Correct specimen-quantity
o   Quantity of specimen required for investigation
§  Blood-3ml-5ml
§  Urine-15ml-20ml
§  Stool- teaspoonful
§  Sputum-5ml
·   Verify information on the request form
o   A request form is used to write down basic information about the patient and to request for a laboratory test. Information required are;
§  Health facility name
§  Hospital Number
§  Name of patient
§  Date requesting
§  Age of the patient
§  Sex of the patient
§  Address of the patient
§  Ward Number
§  Clinical Notes
§  Laboratory investigation
·     Verification of the Specimen Label
o   Hospital Number
o   Name of patient
o   Date requesting
o   Ward Number
o   Name of collector

Documentation of Accessioning Report 

·     Proper entering of information
  • All information should be entered properly in the register book
§  Specimen information
§  Patient information
§  Cause of specimen rejection
§  Action taken

  • Proper handling of information
  • All information should be kept safely for reference
  • Helps in statistics analysis
  • Improves quality of work
o   Proper storage of register books
  • Helps tracking down information
o   Proper use computer program for storing information
§  Use excel program

Key Points 
·         Accessioning of specimens is a process of qualifying specimens for laboratory investigation
·         The following Criteria’s should be adhered during Accessioning of specimens
  • Request form should be well filled with
  • Sufficient quantity of specimen received
  • The specimen should be collected in the right container
  • Assessment of the specimen
·  Criteria’s of Rejecting Specimens
  • Quantity is not sufficient
  • Wrong container
  • Long transport time
  • Wrongly or incomplete filled request form 
  • Condition of the specimen is not ideal for the test (haemolysed blood, dried stool, clots,
  • Unlabelled/Mislabeled specimen
·  Documentation of Accessioning Report

  • All information should be entered properly in the register book
§  Specimen information
§  Patient information
§  Cause of specimen rejection
§  Action taken

  • Proper handling of information
§  All information should be kept safely for reference
§  Helps in statistics analysis
§  Improves quality of work

o   Proper storage of register books
§  Helps tracking down information
o   Proper use computer program for storing information
§  Use excel program
    
Evaluation
·         Explain accessioning of laboratory specimen
·         List four criteria’s of rejecting laboratory specimens
·         List four importance of accessioning of laboratory specimens


References
·         F.J. Baker, R.E. Silverton (2001): Introduction to Medical Laboratory Technology, 7th Edition, Oxford University Press. 
·         Monica Cheesbrough (1981): Medical Laboratory Manual for Tropical Countries Volume I, 2nd Edition Butterworth & Co. (Publishers) Ltd; and
·         Monica Cheesbrough (2002): District Laboratory Practice in Tropical Countries Part 1 & 2, Cambridge University Press.

·         WHO(2003) Manual for Basic Techniques for a health laboratory.2nd Ed. Word Health Organization

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